Tiny Toon Adventures is a cartoon set in the fictional town of Acme Acres, where most of the Tiny Toons and Looney Tunes characters live. The characters attend Acme Looniversity, a school whose faculty primarily consists of the mainstays of the classic Warner Bros. cartoons, such as Bugs Bunny, Daffy Duck, Porky Pig, Sylvester the Cat, Wile E. Coyote and Elmer Fudd. In the series, the university is founded to teach cartoon characters how to become funny. The school is not featured in every episode, as not all of its storylines are centered around the school.
Like the Looney Tunes, the series is derived from cartoon violence (e.g. anvils falling on someone, liberal use of explosives) and slapstick. The series parodies and references the current events of the early 1990s and Hollywood culture. Occasionally, episodes delve into veiled ethical and morality stories of ecology, self-esteem, and crime.